{"action":"create","ckan_id":null,"date_created":"Mon, 01 Sep 2025 08:35:22 GMT","date_finished":null,"harvest_job_id":"499c8608-f73c-4f60-8b8d-5ecf6cd925a2","harvest_source_id":"79e1dbb4-c311-4f5e-9c81-b597afbf383c","id":"a52cba41-39fe-4e14-ade3-dec1793813b2","identifier":"https://data.noaa.gov/waf/NOAA/nmfs/pifsc/iso/xml/64270.xml","parent_identifier":null,"source_hash":"f04cc8877005df50600919d413a3671e070fc032d5d2e929918f9a2bddade35a","source_raw":"\r\n \r\n gov.noaa.nmfs.inport:64270\r\n \r\n \r\n eng\r\n \r\n \r\n UTF8\r\n \r\n \r\n dataset\r\n \r\n \r\n Other\r\n \r\n \r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n \r\n \r\n \r\n \r\n 808-725-5300\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n USA\r\n \r\n \r\n \r\n \r\n \r\n \r\n http://www.pifsc.noaa.gov\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Website\r\n \r\n \r\n Website for this organization\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n 8:00 a.m. - 4:30 p.m.\r\n \r\n \r\n \r\n \r\n resourceProvider\r\n \r\n \r\n \r\n \r\n \r\n \r\n Olenski, Brooke\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n brooke.olenski@noaa.gov\r\n \r\n \r\n \r\n \r\n Email preferred\r\n \r\n \r\n \r\n \r\n pointOfContact\r\n \r\n \r\n \r\n \r\n 2024-02-29T00:00:00\r\n \r\n \r\n ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data\r\n \r\n \r\n ISO 19115-2:2009(E)\r\n \r\n \r\n \r\n \r\n \r\n \r\n EPSG::4326\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across PRIA from 2011 to 2015\r\n \r\n \r\n ARMS Sequencing - PRIA\r\n \r\n \r\n \r\n \r\n 2017\r\n \r\n \r\n creation\r\n \r\n \r\n \r\n \r\n \r\n \r\n 2020-01-07\r\n \r\n \r\n revision\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n publication\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64270\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA\r\n \r\n \r\n \r\n \r\n \r\n 10.7289/v54x563c\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.fisheries.noaa.gov/inport/item/64270\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Full Metadata Record\r\n \r\n \r\n View the complete metadata record on InPort for more information about this dataset.\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n \r\n \r\n \r\n \r\n 808-725-5360\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n \r\n pifsc.info@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.pifsc.noaa.gov\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Pacific Islands Fisheries Science Center Website\r\n \r\n \r\n Pacific Island Fisheries Science Center homepage\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n 8:00 a.m. - 4:30 p.m.\r\n \r\n \r\n \r\n \r\n originator\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://naturalhistory.si.edu/research/global-arms-program\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Global ARMS program\r\n \r\n \r\n Smithsonian partnership - Global ARMS program\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.coris.noaa.gov/monitoring/\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n NOAA's National Coral Reef Monitoring Program\r\n \r\n \r\n Online Resource\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.fisheries.noaa.gov/region/pacific-islands#science\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Information on NOAA PIFSC's official website\r\n \r\n \r\n Online Resource\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n The data described here includes cytochrome oxidase I (COI) DNA metabarcoding data collected from Autonomous Reef Monitoring Structures (ARMS). ARMS were deployed by the NOAA Pacific Islands Fisheries Science Center (PIFSC), Ecosystem Sciences Division (formerly the Coral Reef Ecosystem Division) under the National Coral Reef Monitoring Program (NCRMP) at stationary climate monitoring sites and used to assess and monitor cryptic reef diversity in the Pacific Remote Island Areas (PRIA). Developed in collaboration with the Census of Marine Life (CoML) Census of Coral Reef Ecosystems (CReefs), ARMS were designed to mimic the structural complexity of a reef and attract/collect colonizing marine invertebrates. The key innovation of the ARMS method is that biodiversity is sampled over precisely the same surface area in the exact same manner.\r\n\r\nThese data were gathered at specific reef sites across the PRIAs. ARMS units were set-up, deployed and recovered as described in the ARMS record in the related items section below. After ARMS were disassembled, different size fractions of samples and plate scrapings were preserved in ethanol for metabarcoding.\r\n \r\n \r\n Coral reefs are among the most biologically diverse, complex, and productive of ecosystems. The vast majority of coral reef biodiversity is made up of the small and cryptic organisms living unseen by most within the reef matrix. This hidden community, the cryptobiota, are a critical component of coral reef trophic dynamics and play an essential role in nutrient recycling that enable reefs to thrive in oligotrophic environments. Despite their ecological importance, the cryptobiota are often ignored because they live deep within the reef matrix and require significant taxonomic expertise and time to collect and identify. By leaving ARMS units on the benthos for over two-years to attract the colonization and establishment of the cryptobiota ARMS overcome extraction constraints and by integrating next-generation DNA sequencing into ARMS unit processing, taxonomic limitations are diminshed through the establishment of molecular operational taxonomic units (MOTU) - species proxies - based on DNA sequence similarities.\r\n \r\n \r\n PIFSC Ecosystem Sciences Division and partners, with support from the Census of Marine Life (2008-2010), NOAA's Ocean Acidification Program (2010-2012), the Coral Reef Conservation Program (2012-2018), and the National Science Foundation grant # OISE12343541\r\n \r\n \r\n underDevelopment\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n Email preferred\r\n \r\n \r\n \r\n \r\n custodian\r\n \r\n \r\n \r\n \r\n \r\n \r\n notPlanned\r\n \r\n \r\n These data are merged fasta files of the forward and reverse sequencing on a MiSeq platform and have been deposited into Genbank.\r\n \r\n \r\n \r\n \r\n \r\n \r\n 743\r\n \r\n \r\n National Coral Reef Monitoring Program\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n CRCP Project\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n DNA/RNA Sequencing\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n CoRIS Discovery Thesaurus\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n EARTH SCIENCE > Biosphere > Aquatic Habitat > Benthic Habitat\r\n \r\n \r\n EARTH SCIENCE > Biosphere > Aquatic Habitat > Reef Habitat\r\n \r\n \r\n EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment\r\n \r\n \r\n EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Autonomous Reef Monitoring Structure (ARMS)\r\n \r\n \r\n EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > In Situ Biological\r\n \r\n \r\n EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs\r\n \r\n \r\n EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs > Coral Reef Ecology\r\n \r\n \r\n EARTH SCIENCE > Oceans > Marine Biology > Cryptobiota\r\n \r\n \r\n EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates\r\n \r\n \r\n EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Biodiversity\r\n \r\n \r\n EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Census\r\n \r\n \r\n EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Macroinvertebrates\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n CoRIS Theme Thesaurus\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n INVERTEBRATE SPECIES\r\n \r\n \r\n SPECIES IDENTIFICATION\r\n \r\n \r\n SPECIES IDENTIFICATION - COUNT\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC DATA TYPES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n biological\r\n \r\n \r\n in situ\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC OBSERVATION TYPES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n HI'IALAKAI\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC PLATFORM NAMES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n CORAL REEF STUDIES\r\n \r\n \r\n Coral Reef Conservation Program\r\n \r\n \r\n National Coral Reef Monitoring Program\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC PROJECT NAMES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n US DOC; NOAA; NMFS; Pacific Islands Fisheries Science Center; Ecosystem Sciences Division\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC SUBMITTING INSTITUTION NAMES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n COUNTRY/TERRITORY > United States of America > USA Minor Outlying Islands > Jarvis Island (00S160W0001)\r\n \r\n \r\n COUNTRY/TERRITORY > United States of America > USA Minor Outlying Islands > Kingman Reef (06N162W0001)\r\n \r\n \r\n COUNTRY/TERRITORY > United States of America > USA Minor Outlying Islands > Palmyra Atoll (05N162W0001)\r\n \r\n \r\n COUNTRY/TERRITORY > United States of America > USA Minor Outlying Islands > Wake Atoll (19N167E0001)\r\n \r\n \r\n OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Line Islands > Jarvis Island (00S160W0001)\r\n \r\n \r\n OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Line Islands > Kingman Reef (06N162W0001)\r\n \r\n \r\n OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Line Islands > Palmyra Atoll (05N162W0001)\r\n \r\n \r\n OCEAN BASIN > Pacific Ocean > Central Pacific Ocean > Wake Atoll > Wake Atoll (19N167E0001)\r\n \r\n \r\n place\r\n \r\n \r\n \r\n \r\n CoRIS Place Thesaurus\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Equatorial Pacific Ocean\r\n \r\n \r\n Pacific Remote Islands Marine National Monument\r\n \r\n \r\n place\r\n \r\n \r\n \r\n \r\n NODC Sea Area Names Thesaurus\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS\r\n \r\n \r\n Autonomous Reef Monitoring Structures\r\n \r\n \r\n instrument\r\n \r\n \r\n \r\n \r\n NODC INSTRUMENT TYPES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS\r\n \r\n \r\n Autonomous Reef Monitoring Structure\r\n \r\n \r\n CRED\r\n \r\n \r\n CREP\r\n \r\n \r\n CReef\r\n \r\n \r\n Census of Marine Life\r\n \r\n \r\n Coral Reef Ecosystem Division\r\n \r\n \r\n Coral Reef Ecosystem Program\r\n \r\n \r\n ESD\r\n \r\n \r\n Ecosystem Sciences Division\r\n \r\n \r\n PIFSC\r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n RAMP\r\n \r\n \r\n Reef Assessment and Monitoring Program\r\n \r\n \r\n cytochrome oxidase I\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n \r\n \r\n Triennial\r\n \r\n \r\n temporal\r\n \r\n \r\n \r\n \r\n \r\n \r\n PRIA\r\n \r\n \r\n PRIMNM\r\n \r\n \r\n Pacific Remote Island Areas\r\n \r\n \r\n place\r\n \r\n \r\n \r\n \r\n \r\n \r\n environmental DNA\r\n \r\n \r\n metabarcoding\r\n \r\n \r\n instrument\r\n \r\n \r\n \r\n \r\n \r\n \r\n DOC/NOAA/NMFS/PIFSC > Pacific Islands Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce\r\n \r\n \r\n dataCentre\r\n \r\n \r\n \r\n \r\n Global Change Master Directory (GCMD) Data Center Keywords\r\n \r\n \r\n \r\n \r\n 2017-04-24\r\n \r\n \r\n publication\r\n \r\n \r\n \r\n \r\n 8.5\r\n \r\n \r\n \r\n \r\n Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords\r\n \r\n \r\n GCMD Keyword Forum Page\r\n \r\n \r\n Global Change Master Directory (GCMD). 2024. GCMD Keywords, Version 19. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n custodian\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS\r\n \r\n \r\n project\r\n \r\n \r\n \r\n \r\n InPort\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Cite As: Pacific Islands Fisheries Science Center, [Date of Access]: Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across PRIA from 2011 to 2015 [Data Date Range], https://www.fisheries.noaa.gov/inport/item/64270.\r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.\r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Access Constraints: None\r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Use Constraints: Please cite PIFSC Ecosystem Sciences Division when using the data.\r\n\r\nSuggested citation:\r\n\r\nEcosystem Sciences Division, Pacific Islands Fisheries Science Center, 2020: Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across PRIA from 2011 to 2015, https://www.fisheries.noaa.gov/inport/item/64267.\r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Distribution Liability: While every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.\r\n \r\n \r\n \r\n \r\n \r\n \r\n unclassified\r\n \r\n \r\n Not applicable\r\n \r\n \r\n Not applicable\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across Hawaiian Archipelago from 2010 to 2016\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64268\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across Marianas Archipelago from 2011 to 2014\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64269\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed in Batangas, Philippines from 2012 to 2015\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64332\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n National Coral Reef Monitoring Program: Assessing and Monitoring Cryptic Reef Diversity of Colonizing Marine Invertebrates using Autonomous Reef Monitoring Structures (ARMS) Deployed at Coral Reef Sites across the Pacific Remote Island Areas from 2011 to 2018\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 22465\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n The effects of elevated temperature and acidification on the biodiversity of coral reef cryptobenthic communities that recruited Autonomous Reef Monitoring Structures placed within mesocosms at the Hawaii Institute of Marine Biology between July 2016 and June 2018\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 61283\r\n \r\n \r\n \r\n \r\n \r\n \r\n crossReference\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA Data Management Plan (DMP)\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64270\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/pifsc/dmp/pdf/64270.pdf\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n NOAA Data Management Plan (DMP)\r\n \r\n \r\n NOAA Data Management Plan for this record on InPort.\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n crossReference\r\n \r\n \r\n \r\n \r\n eng; US\r\n \r\n \r\n biota\r\n \r\n \r\n oceans\r\n \r\n \r\n Genbank SRA files:\r\n\r\nGo to: https://www.ncbi.nlm.nih.gov/sra/\r\n \r\n \r\n \r\n \r\n Pacific Remote Island Areas (PRIA) including Baker, Howland, and Jarvis islands, Kingman Reef, and Johnston, Palmyra, and Wake atolls.\r\n \r\n \r\n \r\n \r\n \r\n 166.59824\r\n \r\n \r\n 166.65155\r\n \r\n \r\n 19.27116\r\n \r\n \r\n 19.31654\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Wake Atoll, one of the seven PRIA, is routinely surveyed as part of the Mariana Archipelago RAMP (MARAMP) missions.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n -162.3843\r\n \r\n \r\n -159.99086\r\n \r\n \r\n -0.3818\r\n \r\n \r\n 6.3947\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Phoenix (Baker and Howland) and Line Islands (Jarvis, Kingman, and Palmyra), and Johnston Atoll. These six of the seven PRIA are routinely surveyed as part of the American Samoa RAMP (ASRAMP) missions (Johnston, Baker and Howland during the first leg of ASRAMP, and Jarvis, Kingman, and Palmyra during the last leg of ASRAMP).\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS across Wake Atoll, deployed in 2011 and recovered in 2014 | Currentness: Ground Condition\r\n 2011-03-23\r\n 2014-03-19\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS across the Pacific Remote Island Areas (excluding Wake Atoll) deployed in 2012 and recovered in 2015 | Currentness: Ground Condition\r\n 2012-05-03\r\n 2015-04-28\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS across Wake Atoll, deployed in 2014 and recovered in 2017 | Currentness: Ground Condition\r\n 2014-03-16\r\n 2017-04-23\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS across the Pacific Remote Island Areas | Currentness: Ground Condition\r\n 2015-04-08\r\n 2018-08-05\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NCRMP is a framework for conducting sustained observations of biological, climate, and socioeconomic indicators at 10 priority coral reefs across the U.S. and its territories. This integrated approach consolidates monitoring of coral reefs under a uniform method in the Pacific, Atlantic, Caribbean, and the Gulf of Mexico for the first time. NCRMP is funded by the CRCP and supported by NOAA Fisheries, NOAA National Centers for Coastal Ocean Science (NCCOS), and many other partners. \r\n\r\nThe Ecosystem Sciences Division at NOAA Fisheries is leading climate monitoring in the U.S. Pacific Islands Region. The climate component of NCRMP in the Pacific provides a comprehensive view of climate change impacts on coral reef ecosystems and helps identify areas of resilience and vulnerability. The key indicators used to identify and monitor climate-driven trends include 1) thermal stress caused by changes in sea temperature, 2) ocean acidification resulting from changes in carbonate chemistry, and 3) ecological impacts by collecting data on coral growth rates, erosion, and community structure to understand the impacts of thermal stress and ocean acidification on the ecosystem. Each year, ESD scientists work closely with CRCP and partners during Pacific RAMP missions to collect data using moored oceanographic (subsurface temperature recorders) and ecological (calcification accretion units [CAUs] and autonomous reef monitoring structures [ARMS]) instruments stationed at fixed sites in the Pacific Ocean, and water samples collected by divers. The in-situ data (along with satellite-based observations) are also used in modeling efforts. Innovative analysis techniques are used to develop products that give fellow scientists, managers, decision makers and the public a better understanding of a region's resources and how they are changing over time.\r\n \r\n \r\n \r\n \r\n \r\n \r\n false\r\n \r\n \r\n eng\r\n \r\n \r\n false\r\n \r\n \r\n \r\n \r\n Entity Attribute Detail Citation\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.ncbi.nlm.nih.gov/sra/\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Entity Attribute Detail URL\r\n \r\n \r\n Entity Attribute Detail URL\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n \r\n \r\n \r\n \r\n 808-725-5360\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n \r\n pifsc.info@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.pifsc.noaa.gov\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Pacific Islands Fisheries Science Center Website\r\n \r\n \r\n Pacific Island Fisheries Science Center homepage\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n 8:00 a.m. - 4:30 p.m.\r\n \r\n \r\n \r\n \r\n distributor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://naturalhistory.si.edu/research/global-arms-program\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Global ARMS program\r\n \r\n \r\n Smithsonian partnership - Global ARMS program\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.coris.noaa.gov/monitoring/\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n NOAA's National Coral Reef Monitoring Program\r\n \r\n \r\n Online Resource\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.fisheries.noaa.gov/region/pacific-islands#science\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Information on NOAA PIFSC's official website\r\n \r\n \r\n Online Resource\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n dataset\r\n \r\n \r\n \r\n \r\n \r\n \r\n Accuracy\r\n \r\n \r\n DNA metabarcoding has inevitable PCR amplification and sequencing biases associated with using universal primers that amplify widely divergent taxa, and the variable rates of evolution within and among phyla that affect sequence clustering with a fixed divergence thresholds. Thus, some organisms may not be represented in the DNA metabarcoding data even though they were present on the plates. See https://onlinelibrary.wiley.com/doi/10.1002/edn3.163 for more details.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Analytical Accuracy\r\n \r\n \r\n Analytical results could be different depending on the bioinformatics used to process the raw sequencing data, however, the overall picture will most likely remain the same.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Bias\r\n \r\n \r\n As stated in the Analytical Accuracy section, bias in the data may result in one's choice of tools to bioinformatically process sequencing data.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Completeness Report\r\n \r\n \r\n All ARMS units that are recovered are disassembled, photographed, and sorted by size. Genetic analysis of ARMS samples may exist for a subset of fractions and locations. Missing fractions/locations could be a result of: 1) ARMS that were not recovered due to logistical constraints of the mission; 2) ARMS that were not found when divers returned to the site; and 3) ARMS fractions that were unsuccessfully extracted, amplified, or sequenced.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Conceptual Consistency\r\n \r\n \r\n ARMS sample biodiversity over precisely the same surface area in the exact same manner. Thus, the use of ARMS is a systematic, consistent, and comparable method for monitoring the cryptobiota community overtime. Three units are deployed at each site to allow for replicate measurements. Divers typically record ARMS metadata into the master Microsoft Access database within a few days of the field operations and/or ARMS processing. QA/QC procedures by the ARMS team and the Cruise Data Manager are typically completed during the field mission.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Autonomous Reef Monitoring Structures (ARMS) are assembled, deployed, recovered, and processed as described in the ARMS record in the related items section below. The sessile organisms, the 100 um and 500 um motile fractions, as well as plate scrapings, undergo DNA metabarcoding using the COI gene as the amplicon marker.\r\n \r\n \r\n \r\n \r\n ARMS Deployment -\r\nARMS platforms are deployed as described in detail in the ARMS record of the related items section below.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS Recovery and Processing -\r\n\r\nARMS units are recovered, initially processed, and documented as described in the ARMS record in the related items section below.\r\n\r\nWhen all of the plate layers in the ARMS unit have been photographed and set aside (in seawater), the seawater from the disassembly tub, photo tray, and rinse bucket is sieved through adjoining 2 mm and 500 um sieve pans and an attachable 100 um mesh hand net. Material collected in the 500 um sieve and 100 um net are bulk preserved into two separate jars. Jars are filled with EtOH and labeled accordingly. The preserved 500 and 100 um sample fractions undergo a decantation process at a later date prior to DNA metabarcoding. All plates from an individual ARMS unit are scrapped en masse. Once all plates have been scraped, all the scrapings are transferred into a blender (Brevill; BBL600XL). The scrapings are blended for 45-60 seconds on maximum power until the sample is homogenized. The sample is then transferred from the blender to a 40 um net. The sample in the net is rinsed with filtered (< 40 um) seawater until all discharge from net is clear (takes ~2 gal). Four ~10 ml samples are preserved in 50 ml falcon tubes with DMSO and 4 ~10 ml samples are preserved in 95% EtOH. These blended preserved samples undergo DNA metabarcoding. The remaining sample is stored in a sterile whirlpak at -20C. Between the processing of each ARMS unit the blender is rinsed in fresh water to remove any remaining homogenate. The blender is then placed in a 10% bleach solution for 15 minutes. Finally all parts thoroughly rinsed with DI water if available or fresh water.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Decantation of the 100 and 500 um fractions:\r\n\r\nNOTE: All equipment used in this step that is not already sterile need to be rinsed with fresh water, placed in 10% bleach solution for 20 minutes (new every day), transferred to Milliq water for 20 minutes (bleach bucket that the milliq water will go in first, and rinse) and then place under a UV light for 20 minutes.\r\n\r\nWear gloves. Take sample out the freezer and let sit to defrost before decantation. Empty container(s) into a 1 L conical flask (that has been bleached, rinsed and UV sterilized). Fill the conical flask with ~300 ml of Milli-Q water. Seal neck of flask with parafilm and shake vigorously for 30 seconds. Make sure to hold the parafilm tightly in place with one hand and place the other hand on the base of the flask.\r\n\r\nWhen finished, immediately carefully pour the liquid through the correct sieve (45 \u03bcm for 100- 500 \u03bcm fraction; 106 \u03bcm for 500 \u03bcm \u2013 2 mm fraction) trying not to pour the more dense sediment into the sieve. Fill flask with another 300 ml of Milli-Q and repeat process. Do this 7 times. The aim is to remove all the less dense biological material from the flask through decantation, while keeping the more dense sediment in the flask.\r\n\r\nCollect the material in the sieve and weigh it using a sterile spatula and falcon tube. Put exactly half of the material in a 50 ml falcon tube, fill the tube with ethanol and freeze as a back-up. Place the other half in a sterile mortar and use a pestle to crush the sample for 1 minute. Collect the sample in a 50 ml falcon tube, using a little ethanol to re-suspend it and fill the tube with 95 % ethanol. This sample is now ready for DNA extraction. Store samples at -20C until extraction.\r\n\r\nFinally, collect the sediment left in the conical flask, using a little Milliq water and pour into the same sieve used in the previous step. Collect the material, weigh it and place in a 50ml falcon tube. Fill with ethanol and freeze at -20C.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n For DNA extraction, amplification, and sequencing methods see - Timmers M, Vicente J, Webb M, Jury C, Toonen RJ (2020) Sponging up diversity: evaluating metabarcoding performance for a taxonomically challenging phylum within a complex cryptobenthic community. Environmental DNA. https://doi.org/10.1002/edn3.163\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n To obtain the sequencing data, go to XXXXXXX which takes you to the National Center for Biotechnology Information (NCBI) which hosts Genbank, a genetic sequence database collection of all publicly available DNA sequences. To download a sequence file, click on the SRA link associated with each entry. Click on the data access tab in the new link and select the highlighted name to download the sequence file.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Once sequences are downloaded, you may choose to conduct the bioinformatics in a numbers of ways based on your preference.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Source Contribution: Protocol\r\n \r\n \r\n \r\n \r\n Autonomous Reef Monitoring Structures (ARMS) Overview\r\n \r\n \r\n \r\n \r\n \r\n Molly Timmers\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://origin-apps-pifsc.fisheries.noaa.gov/cred/survey_methods.php#arms\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n Source Citation URL\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n originator\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Access Constraints: None | Use Constraints: None\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n","source_transform":null,"status":"error"}