{"action":"create","ckan_id":null,"date_created":"Mon, 01 Sep 2025 08:35:21 GMT","date_finished":null,"harvest_job_id":"499c8608-f73c-4f60-8b8d-5ecf6cd925a2","harvest_source_id":"79e1dbb4-c311-4f5e-9c81-b597afbf383c","id":"bd5074ac-527f-4167-b1bb-22feb3908fbe","identifier":"https://data.noaa.gov/waf/NOAA/nmfs/pifsc/iso/xml/61283.xml","parent_identifier":null,"source_hash":"6e5b252bbfa1bc7af2eb60ec4041d8603b9594e2e51c38b0575f30ec6f9048b7","source_raw":"\r\n \r\n gov.noaa.nmfs.inport:61283\r\n \r\n \r\n eng\r\n \r\n \r\n UTF8\r\n \r\n \r\n dataset\r\n \r\n \r\n Other\r\n \r\n \r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n \r\n \r\n \r\n \r\n 808-725-5300\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n USA\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.pifsc.noaa.gov\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Website\r\n \r\n \r\n Website for this organization\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n 8:00 a.m. - 4:30 p.m.\r\n \r\n \r\n \r\n \r\n resourceProvider\r\n \r\n \r\n \r\n \r\n \r\n \r\n Olenski, Brooke\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n brooke.olenski@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n pointOfContact\r\n \r\n \r\n \r\n \r\n 2025-05-15T19:16:16\r\n \r\n \r\n ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data\r\n \r\n \r\n ISO 19115-2:2009(E)\r\n \r\n \r\n \r\n \r\n \r\n \r\n The effects of elevated temperature and acidification on the biodiversity of coral reef cryptobenthic communities that recruited Autonomous Reef Monitoring Structures placed within mesocosms at the Hawaii Institute of Marine Biology between July 2016 and June 2018\r\n \r\n \r\n Climate change effects on coral reef cryptobenthic communities\r\n \r\n \r\n \r\n \r\n 2020-12-10\r\n \r\n \r\n creation\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n publication\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 61283\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.fisheries.noaa.gov/inport/item/61283\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Full Metadata Record\r\n \r\n \r\n View the complete metadata record on InPort for more information about this dataset.\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n \r\n \r\n \r\n \r\n 808-725-5360\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n USA\r\n \r\n \r\n pifsc.info@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.pifsc.noaa.gov\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Pacific Islands Fisheries Science Center Website\r\n \r\n \r\n Pacific Island Fisheries Science Center homepage\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n 8:00 a.m. - 4:30 p.m.\r\n \r\n \r\n \r\n \r\n originator\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.fisheries.noaa.gov/region/pacific-islands#science\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n NOAA Fisheries Pacific Islands Region - Science Overview\r\n \r\n \r\n Online Resource\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Timmers M, Vicente J, Webb M, Jury C, Toonen RJ (2020) Sponging up diversity: evaluating metabarcoding performance for a taxonomically challenging phylum within a complex cryptobenthic community. Environmental DNA. https://doi.org/10.1002/edn3.163.\r\n\r\nTimmers M, Jury C, Vicente J, Webb M, and Toonen RJ (In Review) Coral reef biodiversity shuffles but does not decline under the combined stressors of experimental ocean warming and acidification. PNAS.\r\n\r\nJury CP, Bahr K, Barba E, Brainard R, Dobson KL, Graham A, McLachlan R, Nelson C, Price JT, Rocha de Souza M, Shizuru L, Smith C, Sparagon W, Squair C, Timmers M, Tran T, Vicente J, Webb M, Yamase N, Grottoli A, Toonen RJ. (In Review) Experimental reef communities persist under future ocean acidification and warming. Science.\r\n \r\n \r\n \r\n \r\n The data described here includes cytochrome oxidase I (COI) DNA metabarcoding data collected from modified Autonomous Reef Monitoring Structures deployed in mesocosms at the Hawaii Institute of Marine Biology as part of an Ocean Acidification Program funded project granted to NOAA's Pacific Islands Fisheries Science Center (PIFSC), Ecosystem Science Division (formerly known as the Coral Reef Ecosystem Division). Treatments in this fully factorial mesocosm experiment included present-day pH and temperature (Ambient treatment), ocean acidification (-0.2 pH units - Acidified treatment), ocean warming (+2\u00c2\u00b0C - Heated treatment), and future ocean combined stressors (-0.2 pH units and +2 \u00c2\u00b0C - Acidified-Heated treatment). ARMS were placed in replicated mesocosms for each treatment on July 2016 and removed June 2018. Upon removal, the ARMS units were individually scraped clean, contents homogenized, and 10 grams were subsampled from each unit for COI DNA metabarcoding.\r\n \r\n \r\n To examine the long-term responses of biologically diverse coral reef communities to chronic acidification and warming similar to levels projected to occur toward the end of this century. This was the first laboratory study to evaluate the richness and composition of an entire coral reef community that developed over a multi-annual time frame under predicted future ocean conditions.\r\n \r\n \r\n NOAA Fisheries, Ecosystem Sciences Division and funded by the NOAA Ocean Acidification Program\r\n \r\n \r\n completed\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n USA\r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n pointOfContact\r\n \r\n \r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n USA\r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n custodian\r\n \r\n \r\n \r\n \r\n \r\n \r\n notPlanned\r\n \r\n \r\n These data are fastq files directly from the MiSeq sequencer and are already deposited into Genbank and thus there is no maintenance.\r\n \r\n \r\n \r\n \r\n \r\n \r\n DNA/RNA Sequencing\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n CoRIS Discovery Thesaurus\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Autonomous Reef Monitoring Structure (ARMS)\r\n \r\n \r\n EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > DNA/RNA Sequencing\r\n \r\n \r\n EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs\r\n \r\n \r\n EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs > Coral Reef Ecology > BioDiversity\r\n \r\n \r\n EARTH SCIENCE > Oceans > Marine Biology > Crytobiota\r\n \r\n \r\n EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates > Biodiversity\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n CoRIS Theme Thesaurus\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Biodiversity\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC DATA TYPES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n laboratory analyses\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC OBSERVATION TYPES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n CORAL REEF STUDIES\r\n \r\n \r\n US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC PROJECT NAMES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n US DOC; NOAA; NMFS; Pacific Islands Fisheries Science Center; Ecosystem Sciences Division\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n NODC SUBMITTING INSTITUTION NAMES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n COUNTRY/TERRITORY > United States of America > Hawaii > Honolulu > Coconut Island ( Moku o loe ) (21N157W0005)\r\n \r\n \r\n place\r\n \r\n \r\n \r\n \r\n CoRIS Place Thesaurus\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Coastal Waters of Hawaii\r\n \r\n \r\n place\r\n \r\n \r\n \r\n \r\n NODC SEA AREA NAMES THESAURUS:\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS\r\n \r\n \r\n instrument\r\n \r\n \r\n \r\n \r\n NODC INSTRUMENT TYPES THESAURUS\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n CRED\r\n \r\n \r\n CREP\r\n \r\n \r\n Coral Reef Ecosystem Division\r\n \r\n \r\n Coral Reef Ecosystem Program\r\n \r\n \r\n ESD\r\n \r\n \r\n Ecosystem Sciences Division\r\n \r\n \r\n PIFSC\r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n theme\r\n \r\n \r\n \r\n \r\n \r\n \r\n DOC/NOAA/NMFS/PIFSC > Pacific Islands Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce\r\n \r\n \r\n dataCentre\r\n \r\n \r\n \r\n \r\n Global Change Master Directory (GCMD) Data Center Keywords\r\n \r\n \r\n \r\n \r\n 2017-04-24\r\n \r\n \r\n publication\r\n \r\n \r\n \r\n \r\n 8.5\r\n \r\n \r\n \r\n \r\n Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords\r\n \r\n \r\n GCMD Keyword Forum Page\r\n \r\n \r\n Global Change Master Directory (GCMD). 2024. GCMD Keywords, Version 19. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n custodian\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n ARMS\r\n \r\n \r\n project\r\n \r\n \r\n \r\n \r\n InPort\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Cite As: Pacific Islands Fisheries Science Center, [Date of Access]: The effects of elevated temperature and acidification on the biodiversity of coral reef cryptobenthic communities that recruited Autonomous Reef Monitoring Structures placed within mesocosms at the Hawaii Institute of Marine Biology between July 2016 and June 2018 [Data Date Range], https://www.fisheries.noaa.gov/inport/item/61283.\r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.\r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Access Constraints: None\r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Use Constraints: Please cite NOAA Fisheries, Ecosystem Science Division (ESD) when using the data.\r\n\r\nExample:\r\n\r\nEcosystem Science Division; COPY INPORT RECORD CITATION HERE (keep ESD).\r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Distribution Liability: While every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.\r\n \r\n \r\n \r\n \r\n \r\n \r\n unclassified\r\n \r\n \r\n Not applicable\r\n \r\n \r\n Not applicable\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across American Samoa from 2012 to 2015\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64267\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across Hawaiian Archipelago from 2010 to 2016\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64268\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across Marianas Archipelago from 2011 to 2014\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64269\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed at Coral Reef Sites across PRIA from 2011 to 2015\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64270\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Cryptobiota metabarcoding using Autonomous Reef Monitoring Structure (ARMS) Deployed in Batangas, Philippines from 2012 to 2015\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 64332\r\n \r\n \r\n \r\n \r\n \r\n \r\n series\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA Data Management Plan (DMP)\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n NOAA/NMFS/EDM\r\n \r\n \r\n \r\n \r\n \r\n 61283\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/pifsc/dmp/pdf/61283.pdf\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n NOAA Data Management Plan (DMP)\r\n \r\n \r\n NOAA Data Management Plan for this record on InPort.\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n crossReference\r\n \r\n \r\n \r\n \r\n eng; US\r\n \r\n \r\n biota\r\n \r\n \r\n climatologyMeteorologyAtmosphere\r\n \r\n \r\n Genbank SRA files: SRS7105074 \u2010 SRS7105095\r\nGo to: https://www.ncbi.nlm.nih.gov/sra/\r\n \r\n \r\n \r\n \r\n Coconut Island ( Moku o loe ) (21N157W0005)\r\n \r\n \r\n \r\n \r\n \r\n -155.06861111111\r\n \r\n \r\n -155.05861111111\r\n \r\n \r\n 19.72944444444\r\n \r\n \r\n 19.73944444444\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Hawaii Institute of Marine Biology on Coconut Island in Kaneohe Bay within the island of Oahu.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n This represents the time frame in which the ARMS remained within the mesocosm treatments. | Currentness: UPDATE CURRENTNESS TO REFLECT WHAT TIME FRAME DATES REPRESENT\r\n 2016-07-06\r\n 2018-06-14\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n false\r\n \r\n \r\n eng\r\n \r\n \r\n false\r\n \r\n \r\n \r\n \r\n Entity Attribute Detail Citation\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.ncbi.nlm.nih.gov/sra/\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Entity Attribute Detail URL\r\n \r\n \r\n Entity Attribute Detail URL\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n FASTQ\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Pacific Islands Fisheries Science Center\r\n \r\n \r\n \r\n \r\n \r\n \r\n 808-725-5360\r\n \r\n \r\n \r\n \r\n \r\n \r\n 1845 Wasp Blvd.\r\n \r\n \r\n Honolulu\r\n \r\n \r\n HI\r\n \r\n \r\n 96818\r\n \r\n \r\n USA\r\n \r\n \r\n pifsc.info@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.pifsc.noaa.gov\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Pacific Islands Fisheries Science Center Website\r\n \r\n \r\n Pacific Island Fisheries Science Center homepage\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n 8:00 a.m. - 4:30 p.m.\r\n \r\n \r\n \r\n \r\n distributor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.ncbi.nlm.nih.gov/biosample?LinkName=bioproject_biosample_all&from_uid=649058\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Illumina Sequences\r\n \r\n \r\n Raw illumina sequences are available in NCBI SRA (Sequence Read Archive, Accession: SRS7105074\u2010SRS7105095. These accession numbers represent 22 samples and includes the forward and reverse fastq file for each sample. Metabarcoding data was submitted to Genebank via Genome - https://geome-db.org/ which tags and records metadata associated with sequence files.\r\n \r\n \r\n download\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n dataset\r\n \r\n \r\n \r\n \r\n \r\n \r\n Accuracy\r\n \r\n \r\n DNA metabarcoding has inevitable PCR amplification and sequencing biases associated with using universal primers that amplify widely divergent taxa, and the variable rates of evolution within and among phyla that affect sequence clustering with a fixed divergence thresholds. Thus, some organisms may not be represented in the DNA metabarcoding data even though they were present on the plates. See https://onlinelibrary.wiley.com/doi/10.1002/edn3.163 for more details.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Analytical Accuracy\r\n \r\n \r\n Analytical results could be different depending on the bioinformatics used to process the raw sequencing data, however, the overall picture will most likely remain the same.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Bias\r\n \r\n \r\n As stated in the Analytical Accuracy section, bias in the data may result in one's choice of tools to bioinformatically process sequencing data.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Comparability\r\n \r\n \r\n If the reader follows the direct bioinformatic processing steps as outlined in Timmers et al. XXX, the data will be comparable.\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Completeness Report\r\n \r\n \r\n See Timmers et al. XXX\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Conceptual Consistency\r\n \r\n \r\n See Timmers et al. XXX\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n These data represent the first laboratory study to evaluate the richness and composition of an entire coral reef community that developed over a multi-annual time frame under predicted future ocean conditions.\r\n \r\n \r\n \r\n \r\n For mesocosm set-up, ARMS processing, DNA extraction, amplification, and sequencing see Timmers M, Jury C, Vicente J, Webb M, and Toonen RJ (In Review) Coral reef biodiversity shuffles but does not decline under the combined stressors of experimental ocean warming and acidification. PNAS.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n To obtain the sequencing data from this study, go to https://www.ncbi.nlm.nih.gov/biosample?LinkName=bioproject_biosample_all&from_uid=649058 which takes you to the National Center for Biotechnology Information (NCBI) which hosts Genebank, a genetic sequence database collection of all publicly available DNA sequences. To download a sequence file, click on the SRA link associated with each entry. Click on the data access tab in the new link and select the highlighted name to download the sequence file.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Once sequences are downloaded, you may choose to conduct the bioinformatics in a numbers of ways based on your preference. To process the samples in the same way as was conducted by NOAA, please refer to the supplementary information in the paper, Coral reef biodiversity shuffles but does not decline under the combined stressors of experimental ocean warming and acidification.\r\n \r\n \r\n \r\n \r\n Timmers, Molly A\r\n \r\n \r\n \r\n \r\n \r\n \r\n (808)725-5449\r\n \r\n \r\n \r\n \r\n \r\n \r\n molly.timmers@noaa.gov\r\n \r\n \r\n \r\n \r\n \r\n \r\n processor\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n Biodiversity of coral reef cryptobiota shuffles but does not decline under the combined stressors of ocean warming and acidification\r\n \r\n \r\n \r\n \r\n 2021-09-28\r\n \r\n \r\n publication\r\n \r\n \r\n \r\n \r\n \r\n \r\n Molly Timmers\r\n \r\n \r\n \r\n \r\n \r\n \r\n https://www.pnas.org/content/118/39/e2103275118\r\n \r\n \r\n WWW:LINK-1.0-http--link\r\n \r\n \r\n Source Citation URL\r\n \r\n \r\n Source Citation URL\r\n \r\n \r\n information\r\n \r\n \r\n \r\n \r\n \r\n \r\n originator\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n otherRestrictions\r\n \r\n \r\n Access Constraints: None | Use Constraints: None\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n","source_transform":null,"status":"error"}